脑出血（Intracerebral haemorrhage, ICH）在我国每年的发病人数约为50-100万，由于其高致死率（28d内约49.4%）和致残率（80%），已是我国居民死亡和残疾的主要原因之一。外科手术作为治疗ICH的重要手段之一，对于有大量血肿的严重颅高压甚至脑疝的患者，手术治疗在拯救生命方面具有肯定的作用。但外科手术治疗ICH的价值在国际上仍存在诸多争议，主要原因是外科手术与保守治疗相比患者的长期预后并未得到改善。因此如何提高外科手术治疗的预后是当前ICH治疗的一个亟待解决的关键问题。
本研究提出构建载铁离子螯合剂的角蛋白止血凝胶用于脑出血的术后植入，发现其植入后可快速降低脑内局部的铁离子浓度，进而可降低动物的脑出血损害及神经功能恢复。该论文已发表在International Journal of Pharmaceutics杂志，该论文由罗甜甜硕士、郭廷旺博士、杨倩硕士共同完成。
In Situ Hydrogels Enhancing Postoperative Functional Recovery by Reducing Iron Overload after Intracerebral Haemorrhage
Luo T, Guo T, Yang Q, Hao S *, Wang J, Cheng Z, Qu Q, He Y, Gong Y, Gao F, Li W, Xia H, Wang B *.
The role of surgery for most patients with spontaneous intracerebral haemorrhage (ICH) remains controversial due to the continuous occurrence of postoperative iron overload induced by low clot clearance rate. In this study, human hair keratose hydrogel (KG) loading with minocycline hydrochloride (MH) were prepared to reduce iron overload for the improvement of the postoperative functional recovery after ICH aspiration surgery. Hemoglobin-induced iron accumulation in rat primary neuronal culture was delayed by the adsorptive capacity of blank KG, while MH-loaded KG displayed a stronger and more thorough cytoprotective effect than blank KG due to the combined effect of absorptive action to iron and sustained release of the iron chelator. Moreover, high iron-chelating efficiency in the hematoma region supplied by MH-loaded KG significantly reduced dose strength of iron chelator. In situ injection of KG with different MH loadings (2, 20, and 200μg) into the hematoma region after aspiration surgery showed a stronger effect on the reduction of ICH-induced iron accumulation, edema, and neurological deficits in rats compared to the postoperative intraperitoneal administration of MH (approximately 15mg). These results suggested that the in situ KG not only could effectively reduce the ICH postoperative iron overload and improve the postoperative functional recovery via the iron adsorption and sustained release of MH, but also has great potential to reduce the systemic adverse effects by decreasing the dose strength of iron chelator.